Table 1.

Immunological parameters before and 6 months post-hematopoietic stem cell transplantation (HSCT).

	Before HSCT	6 months post HSCT
IgG (mg/dl)	52	571
IgA (mg/dl)	<5	57
IgM (mg/dl)	<5	51
CD4+ (c/μl)	1,840	818
CD8+ (c/μl)	450	613
CD4+/CD8+	4.1	1.3
CD20+ (c/μl)	1,329	588
CD20+IgD+CD27− (c/μl)	1,329	588
CD20+IgD−CD27+ (c/μl)	0	7
CD56+ (c/μl)	208	148
CD14+ (c/μl)	913	292
CD66b+ CD49d− (c/μl)	2,040	3,723
Chimerism (BM) (% autologous portion)	–	1–5
Chimerism (blood) (% autologous portion)	–	2.9

Immunoglobulins (Ig) G, A, A were measured in the serum by ELISA. T helper cells (CD4+), cytotoxic T cells (CD8+), B cells (CD20+), naïve B cells (CD20+IgD+CD27−), memory B cells (CD20+IgD− CD27+), NK-cells (CD56+), monocytes (CD14+), neutrophilic granulocytes (CD66b+CD49d−) were measured by flow cytometry. Chimerism analysis in bone marrow (BM) and blood was performed using molecular genetic methods or XY-Fish, respectively.