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. 2017 Apr 13;8(2):14. doi: 10.3390/jfb8020014

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Microvessels of a rat’s iris viewed under a magnifying glass before and after an injection of Hb-Vs [1] (a-1) Before the injection; (a-2) The specific area in the rat’s iris before the injection; (a-3) Two-dimensional changes in the microvessels were analyzed by area extraction using simple binarizing image processing (Image J, National Institutes of Health, Maryland, USA); (b-1) After the injection of Hb-Vs, showing a marked expansion in the microvessels, with the microvessel area in the bird’s-eye view increasing by 20%; (b-2) The specific area after the injection; (b-3) Two-dimensional changes in the microvessels were analyzed by area extraction using simple binarizing image processing.