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Absorbance at 595 nm in heparinized human blood dispersed in normal saline, Hb-Vs dispersed in normal saline, and a 1:1 mixture of blood and Hb-Vs in saline [1]. Each 100% concentration sample contained 10 g/dL Hb. Ultrospec 3000 UV/Visible Spectrophotometer (Pharmacia Biotech, New Jersey, USA; wavelength accuracy ± 1 nm; photometric accuracy ± 0.5% or ±0.003–3.000 A, whichever was the greater; light path 10 mm) was used for absorbance measurement.
