Table 1.

Statistical analysis results of the challenge with P. berghei expressing P. vivax TRAP. As shown in this table, all groups vaccinated with protein plus adjuvant and/or VLP show significantly increased survival compared to the group vaccinated with PBS (naïve). However, when we compare naïve vs. only protein, there is no statistical difference. When comparing mice vaccinated with only protein with protein plus alum adjuvants, there was no statistical difference in survival, but if those groups are compared with MCT and or VLP, there is a significant difference in survival. The group vaccinated with PvTRAP-CMVttVLP + MCT adjuvant exhibits significantly better protection compared with all other groups. Data of six mice per group were analysed using GraphPad Prism software applied to assess the means of six groups by Tukey’s multiple comparison test of one-way ANOVA. ns = not significant, *** = p value < 0.0001; ** = p value < 0.001; * = p value < 0.01.

Tukey‘s Multiple Comparison Test	Significant Summary
Naïve vs. Only PvTRAP	ns
Naïve vs. PvTRAP + Alum	*
Naïve vs. PvTRAP + MCT	**
Naïve vs. PvTRAP-CMVttVLP	***
Naïve vs. PvTRAP-CMVttVLP + MCT	***
Only PvTRAP vs. PvTRAP + Alum	ns
Only PvTRAP vs. PvTRAP + MCT	*
Only PvTRAP vs. PvTRAP-CMVttVLP	**
Only PvTRAP vs. PvTRAP-CMVttVLP + MCT	**
PvTRAP + Alum vs. PvTRAP + MCT	ns
PvTRAP + Alum vs. PvTRAP-CMVttVLP	**
PvTRAP + Alum vs. PvTRAP-CMVttVLP + MCT	***
PvTRAP+MCT vs. PvTRAP-CMVttVLP	*
PvTRAP+MCT vs. PvTRAP-CMVttVLP + MCT	**
PvTRAP-CMVttVLP vs. PvTRAP-CMVttVLP + MCT	*