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. 2017 May 17;5(2):11. doi: 10.3390/vaccines5020011

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Size-exclusion chromatography (SE-HPLC) chromatograms for NIC7 conjugates made with high and low ratios of sulfo N-hydroxysuccinimide (sNHS) and 1-ethyl-3-(3-dimethylamino)propyl carbodiimide hydrochloride (EDC). The top panel shows the SE-HPLC profile for a conjugate produced with a sNHS/EDC ratio of 0.14. For this sample, the amount of Main 1 vs. Main 2 is almost 1:1. The middle panel shows the SE-HPLC profile for a conjugate produced with a sNHS/EDC ratio of 0.86 and Main 2 is present to a much lower extent. The bottom panel shows a control sample of unconjugated CRM₁₉₇ showing that Main 2 is not present.