Figure 4.
Effect of different sNHS/EDC ratios on antibody (Ab) titers. BALB/c mice (n = 10/group) were immunized on Days 0 and 21 by IM injection with 10 µg of different NIC7-CRM conjugates adjuvanted with alum (10 µg Al3+) + CpG (20 µg). Plasma was collected on Day 20 and 28 and anti-nicotine antibody levels determined by ELISA. Anti-nicotine Ab titers post-Dose 1 and 2 are shown in Panels (A) and (B), respectively. Statistical significance was calculated by a one-factor analysis of variance (ANOVA) followed by Tukey’s post-hoc multiple comparison test. * p < 0.05.