Figure 5.

The effect of different sNHS/EDC ratios on Ab function. BALB/c mice (n = 10/group) were immunized on Days 0 and 21 by IM injection with 10 µg of NIC7-CRM conjugates adjuvanted with alum (10 µg Al³⁺) + CpG (20 µg). On Day 28, animals received an IV injection of ³H-nicotine (0.05 mg/kg) and plasma and brains collected. Panel (A) shows % reduction of nicotine levels in brain (ng-eq/g), and Panel (B) shows % increase of nicotine levels in plasma (ng-eq/mL) compared to control non-immunized mice. * p < 0.05; ** p < 0.01; **** p < 0.