Figure 3.

The lack of a mobility effect upon microinjection of lamin A/C histone-binding site antibodies is observed for both euchromatic and heterochromatic regions. (a) An HT1080 cell line with a 254 copy LacO array integrated into a euchromatic region of chromosome 5 that tended to be in the nuclear interior was microinjected with lamin A/C histone-binding site antibodies. At 3 h post-injection, the cell was imaged live for 70 min with Z-stacks taken every 10 min. For comparison, a non-injected cell in the same field is shown in the bottom panels. No visual increase in mobility was observed in this or any of several other injected cells observed. (b) A similar array integrated into a heterochromatic region of HT1080 chromosome 13 that tended to be at the nuclear periphery was similarly analysed, except that imaging was started at 1 h post-injection and continued for 3 h. Arrowheads indicate the LacO array in the bottom movie, as the spot is more difficult to distinguish in this movie. Again, no difference was observed between the injected and non-injected cells, and very little mobility was observed at all. Scale bars, 10 µm.