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Cytochrome P450s from H. halys (126 sequences) and M. histrionica (86 sequences) plus two from Rhodnius prolixus and one from Riptortus pedestris were aligned using Clustal Omega (http://www.ebi.ac.uk/Tools/msa/clustalo/) at EBI. An NJ midpoint-rooted tree was made. The tree was drawn using Figtree v1.3.1 and labeled in Adobe Illustrator CC ver17.0.0. P450 clans are colored. Sequences less than 175 amino acids were not included.
