Table 3.
Gold-tier transcripts exhibiting the greatest gene expression fold changes within each of the three harlequin bug mRNA population comparisons performed. Expression levels were conveyed using the Transcripts Per Million (TPM) measure, and binary logarithms were used to rank sample-specific ratios of these amounts. A floor on TPM values of 5.0 was imposed.
| 2nd Instar Expression Relative to 4th Instar | ||||
| log2FC | Direction | 2nd Instar | 4th Instar | NR Gene |
| 5.1284 | up | 253.24 | 7.24 | adult-specific cuticular protein ACP-20-like |
| 4.6366 | up | 1055.89 | 42.45 | uncharacterized protein LOC106688964 |
| 3.6726 | up | 366.36 | 28.73 | tubulin beta-1 chain |
| 3.5047 | up | 380.01 | 33.48 | protein takeout-like |
| 3.3727 | up | 192.25 | 18.56 | GTP cyclohydrolase 1 isoform X1 |
| 5.3563 | down | 8.17 | 334.68 | heat shock 70 kDa protein cognate 4 |
| 5.3279 | down | 7.17 | 287.99 | acyl-CoA Delta(11) desaturase-like isoform X1 |
| 5.2056 | down | 6.33 | 233.59 | uncharacterized protein LOC106679388 |
| 4.7751 | down | 9.21 | 252.18 | lysosomal alpha-mannosidase |
| 4.6475 | down | 6.05 | 151.63 | uncharacterized protein LOC106683993 |
| Female Expression Relative to Male | ||||
| log2FC | Direction | Female | Male | NR Gene |
| 4.1357 | up | 375.11 | 21.34 | heat shock 70 kDa protein cognate 4 |
| 3.5908 | up | 104.22 | 8.65 | JH acid O-methyltransferase-like isoform X1 |
| 2.8439 | up | 88.45 | 12.32 | uncharacterized protein LOC106679932 |
| 2.7760 | up | 124.18 | 18.13 | troponin C, isoform 1-like |
| 2.7262 | up | 34.21 | 5.17 | adult-specific cuticular protein ACP-20-like |
| 3.9320 | down | 570.39 | 8706.01 | putative odorant-binding protein 4 |
| 2.7080 | down | 5.58 | 36.46 | E3 ubiquitin-protein ligase TRIM37-like isoform X2 |
| 2.2543 | down | 6.29 | 30.01 | exocyst complex component 7 |
| 1.9412 | down | 6.51 | 25.00 | DDB1- and CUL4-associated factor 6-like |
| 1.9045 | down | 7.18 | 26.88 | exosome complex component CSL4 |
| Adult Expression Relative to Nymph | ||||
| log2FC | Direction | Adult | Nymph | NR Gene |
| 3.8701 | up | 5288.23 | 361.66 | putative odorant-binding protein 4 |
| 3.7040 | up | 105.04 | 8.06 | inositol monophosphatase 2-like |
| 3.4889 | up | 98.80 | 8.80 | uncharacterized protein LOC106686819 |
| 3.4091 | up | 78.93 | 7.43 | uncharacterized protein LOC106691169 |
| 3.0628 | up | 264.22 | 31.62 | NADP-dependent malic enzyme-like isoform X3 |
| 6.0853 | down | 44.80 | 3041.89 | uncharacterized protein LOC106688633 |
| 4.3486 | down | 5.41 | 110.22 | pro-resilin-like |
| 3.7376 | down | 13.83 | 184.48 | probable antibacterial peptide |
| 3.6876 | down | 9.00 | 115.96 | cuticle protein 18.6, isoform B |
| 3.2451 | down | 11.07 | 104.96 | GTP cyclohydrolase 1 isoform X1 |