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. 2017 Jun 9;8(2):60. doi: 10.3390/insects8020060

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Egg maturation and female mating frequency by age. (A–C) The ovary of 1-, 2-, and 3-day-old females; scale in lower left is equal to 2.0 mm. After feeding, the ovary gradually developed in the body, and there were some mature eggs in ovaries of 3-day-old females. (D) The mean number of moth pairs observed copulating each day (means with the same latter are not significantly different; p > 0.05, one-way ANOVA with Duncan’ test). Error bars signify SE. Nearly all moths copulated at the age of 3 days or older.