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. 2017 Jun 30;8:458. doi: 10.3389/fphys.2017.00458

Figure 1.

Figure 1

Experimental design of the present study showing from top to bottom: (i) Developmental stages considered (larvae and juvenile); (ii) Days post hatch (0, 3, 10, 20, 41, and 55); (iii) Main events during larval development (hatch, mouth opening, pre-metamorphosis, pro-metamorphosis, post-metamorphosis); (iv) Experimental groups listed on the left column, graphically depicted in central columns: Control, larvae fed rotifers and Artemia metanauplii enriched with Easy Selco (ES); PreVA10, larvae fed rotifers enriched with ES supplemented with a dietary content 10 fold above normal (VA10) and afterwards Artemia metanauplii enriched with ES; PreVA50, larvae fed rotifers enriched with ES supplemented with a dietary content 50 fold above normal (VA50) and afterwards Artemia metanauplii enriched with ES; ProVA10, larvae fed rotifers enriched with ES, Artemia metanauplii enriched with ES supplemented with a dietary content in 10 fold above normal (VA10) during pro-metamorphosis (from 10 to 20 dph) and afterwards Artemia metanauplii enriched with ES; ProVA50, larvae fed rotifers enriched with ES, Artemia metanauplii enriched with ES supplemented with a dietary content in 50 fold above normal (VA50) during pro-metamorphosis (from 10 to 20 dph) and afterwards Artemia metanauplii enriched with ES; PostVA10, larvae fed rotifers enriched with ES, with Artemia metanauplii enriched with ES (from 10 to 20 dph) and afterwards Artemia metanauplii enriched with ES supplemented with a dietary content in 10 fold above normal (VA10) during post-metamorphosis (from 21 to 41 dph); PostVA50, larvae fed rotifers enriched with ES, with Artemia metanauplii enriched with ES (from 10 to 20 dph) and afterwards Artemia metanauplii enriched with ES supplemented with a dietary content in 50 fold above normal (VA50) during post-metamorphosis (from 21 to 40 dph); and weaning process for all experimental groups on the right column from 41 to 55 dph.