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. 2017 Jun 30;8:458. doi: 10.3389/fphys.2017.00458

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Copyright © 2017 Fernández, Ortiz-Delgado, Darias, Hontoria, Andree, Manchado, Sarasquete and Gisbert.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Semi-quantitative assessment of triiodothyronine (T3) hormone (mean ± standard deviation) content by using immunohistochemical approaches in Senegalese sole fed with experimental diets. T3 content at 10 (A), 21 (B), and 55 (C) dph. A detailed view of the immunoreactive intensity in thyroid follicles in a Control (D), PreVA10 (E), and PreVA50 (F) larvae at 10 dph. CC, colloid content; CV, cortical vesicles content; EC, epithelial cells content. For a detailed description about the different experimental groups, please see the legend of Figure 1. Different letters at the top of each bar denotes statistically significant differences among experimental groups (ANOVA, P < 0.05; N = 3). Scale bar = 200 μm.