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. 2005 Feb 7;2:6. doi: 10.1186/1742-4690-2-6

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Copyright © 2005 Epie et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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LIS1 interacts with Tat in yeast two-hybrid assay. EGY48 yeast cells were transformed, as described in Experimental procedures, with pSH18–34 reporter and combinations of pLexA and pJG4–5 empty vectors (panel A); pLexA and pJG LIS1 (panel B); pLexA Tat and pJG 4–5 (panel C); pLexA Tat 86 and pJG LIS1 (panel D). Six independent colonies from each transformation were cultured on plates containing Galactose/Raffinose to induce Tat and LIS1 synthesis and X-Gal substrate to detect β-galactosidase.