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. 2017 Jun 29;37(14):e00632-16. doi: 10.1128/MCB.00632-16

FIG 2.

FIG 2

Upregulation of Sox8 upon Wnt3a conditioned medium treatment in Gc1-Spg and TM4 cells. (A) Expression and localization of Sox8 in Gc1-Spg and TM4 cell lines. (B) Immunofluorescence analysis in Gc1-Spg cells upon Sox8 silencing to show the specificity of the antibody used. (C) Expression analysis of Sox8 and Sox9 in Wnt3A conditioned medium-treated Gc1-Spg and TM4. Ctrl, control. (D) Luciferase assay for Sox8 and Sox9 promoter in control and Wnt3A conditioned medium-treated Gc1-Spg cells. (E) Western blot showing upregulation of Sox8 in Wnt3A conditioned medium-treated Gc1-Spg and TM4 cells. Data in panels C and D are plotted as means ± SD (n = 4). **, P ≤ 0.01; *, P ≤ 0.05 (two-tailed Student t test). Bar (A and B), 25 μm.