Analysis of the role of mrhl RNA in Wnt-mediated upregulation of Sox8. (A) Expression analysis of mrhl RNA and Sox8 in control and Wnt3A conditioned medium-treated Gc1-Spg upon mrhl RNA overexpression (OE) and mrhl RNA silencing (si). pcDNA, vector control. (B) Luciferase assay for the Sox8 promoter in control and Wnt3A conditioned medium-treated Gc1-Spg upon mrhl RNA overexpression and mrhl RNA silencing. Data are plotted as means ± SD, n = 4. ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05 (two-tailed Student t test).