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. 2017 Jun 29;37(14):e00632-16. doi: 10.1128/MCB.00632-16

FIG 6.

FIG 6

Sin3A is bound at the Myc-Max-Mad binding site on the Sox8 promoter to keep it in a repressed state. (A) ChIP showing the presence of Max at the Myc-Max-Mad binding site on the Sox8 promoter in both control and Wnt3A conditioned medium-treated Gc1-Spg cells. (B) ChIP in Gc1-Spg cells with Zfp113 as a positive control, showing the occupancy of Sin3A in control Gc1-Spg cells but not in Wnt3A conditioned medium-treated Gc1-Spg cells. The region from bp −500 to −700 in the Sox8 promoter is used as a negative control. (C) Sin3A ChIP upon mrhl RNA silencing. (D) ChIP showing the presence of HDAC1 at the Myc-Max-Mad binding site on the Sox8 promoter in control Gc1-Spg cells but not in Wnt3A conditioned medium-treated Gc1-Spg cells. Data are plotted as means ± SD, n = 4. ***, P ≤ 0.001; **, P ≤ 0.01; *, P ≤ 0.05 (two-tailed Student t test). N. S, not significant.