FIG 8.

Role of Sox8 and Wnt signaling in meiotic progression and commitment of spermatogonial cells. (A) Sox8 binding site in the 1-kb upstream promoters of important premeiotic and meiotic marker genes. (B) Expression analysis of Sox8 by RT-qPCR and Western blotting upon Sox8 silencing by shRNA. (C) Expression analysis of premeiotic markers (Stra8, Lhx8, and c-kit) and meiotic markers (Mtl5 and Hspa2) upon Wnt signaling activation as well as upon Sox8 silencing in control and Wnt3A conditioned medium-treated Gc1-Spg cells. (D) Change in global chromatin architecture in mouse spermatogonial Gc1-Spg cells upon treatment with Wnt3A conditioned medium for different time durations (2, 4, and 6 days). (E) Apoptosis assay of Gc1-Spg cells upon prolonged Wnt3A treatment (24, 48, 72, and 96 h). PE, R-phycoerythrin.