TABLE 1.
List of primers used for RT-qPCR, ChIP-qPCR, and nucleosome mapping (NM1 to NM19)
| Gene or locus (purpose) | Forward primer | Reverse primer |
|---|---|---|
| Actba (RT-qPCR) | AGGTCATCACTATTGGCAACG | TACTCCTGCTTGCTGATCCAC |
| Sox9 (RT-qPCR) | TGCTGAACGAGAGCGAGAAGAGAC | GGACCCTGAGATTGCCCAGAGTG |
| Sox8 primary (RT-qPCR) | CTGTGTTTTCTGTAGCTTGCTG | CCTGGAGCCCACCTGTGTG |
| Sox8 (RT-qPCR) | GCTGTGGCGCTTGCTGAG | CTGTGTGGTGGTCACTGTG |
| Mrhl (RT-qPCR) | TGAGGACCATGGCTGGACTCT | AGATGCAGTTTCCAATGTCCAAAT |
| c-Myc (RT-qPCR) | CTAGTGCTGCATGAGGAGAC | CTCTTGGCAGCTGGATAGTC |
| Max (RT-qPCR) | GATAACGATGACATCGAGGTG | TTGCTCCAGAAGAGCATTCTG |
| Mad (RT-qPCR) | CTGTCCACCAAATAGACCAG | TGCATGCTGCCTCGCTCG |
| Stra-8 (RT-qPCR) | CGTGGCAAGTTTCCTGGACAAG | GGCTCTGGTTCCTGGTTTAATGG |
| Lhx8 (RT-qPCR) | GTCTGGAGATAGTTGGCTCGAGT | GGATGGTAGGCTTTGTAAACTAG |
| c-Kit (RT-qPCR) | CCCGACGCAACTTCCTTA | CGCTTCTGCCTGCTCTTC |
| Mtl-5 (RT-qPCR) | CGTCTGGGAGCTGCTAAA | GGAGGTCCTGAGAACTTGG |
| Hspa2 (RT-qPCR) | CTACGTGGCCTTCACTGACA | GGTCAGGATGGACACATCGA |
| Actb (ChIP-qPCR) | TCCACAAGGGCGGAGGCTAT | GGGTTTTATAGGACGCCACA |
| Ccnd1 (ChIP-qPCR) | CCATTCTCCCGGTTTAAGAACAG | AGCCTTCGTAGATATGCAAATCG |
| Cad (ChIP-qPCR) | CACACAAGACTTCCAGCTCAC | TGTAGTCAATAATACAAATTAGTAC |
| NM1 | GATCCCAGACCTGAAGGTTAG | GTGGTCTCTAATATCTCCATCTC |
| NM2 | GAGATGGAGATATTAGAGACCAC | ACCTGTTTCCCAAGCCCGTG |
| NM3 | CACGGGCTTGGGAAACAGGT | CTTGTCTGGGTGGCATAGAG |
| NM4 | CTCTATGCCACCCAGACAAG | CGAAACTCAAAGGAGGCGATG |
| NM5 | CATCGCCTCCTTTGAGTTTCG | GAGGCGAGAGCCAAGCCTG |
| NM6 | CAGGCTTGGCTCTCGCCTC | CCTAGAGAGAAGGCGGAGAG |
| NM7 | CTCTCCGCCTTCTCTCTAGG | CTGGGTGGTCTGGCGGAG |
| NM8 | CTCCGCCAGACCACCCAG | GTCCTTCCGGGTATGACCTG |
| NM9 | CAGGTCATACCCGGAAGGAC | CTGGCAGTGACTGCTGAGTC |
| NM10 | GACTCAGCAGTCACTGCCAG | GCTAGACAGAGGTGGGAGG |
| NM11 | CCTCCCACCTCTGTCTAGC | CCAGGTGACCCTGGTAACTC |
| NM12 | GAGTTACCAGGGTCACCTGG | GCAACCAAACCCGGGTTCTG |
| NM13 | CAGAACCCGGGTTTGGTTGC | AAGGTTCCTGAGTCCAACATC |
| NM14 | GATGTTGGACTCAGGAACCTT | GCAGCACGGCTTGACTTTCG |
| NM15 | CGAAAGTCAAGCCGTGCTGC | GGTCAGAGGGCTAAGGGTG |
| NM16 | CACCCTTAGCCCTCTGACC | TCCGTACCACGTGGGCCAG |
| NM17 | TGGCCCACGTGGTACGGAG | AATCCAAAGAAGCCTTGCGAC |
| NM18 | TCGCAAGGCTTCTTTGGATTC | TCCGGGTTGGCAGGTCCTC |
| NM19 | AGGACCTGCCAACCCGGAC | CAGTTCTGGGACCCGCAAC |
| Mutant Tcf4 binding site | GTACATCGCCTCCGCTGAGTTTCGCGGGG | CCCCGCGAAACTCAGCGGAGGCGATGTAC |
| Zfp113 (ChIP-qPCR) | ACCACAACTTCCACCTCACC | GGGTCTGTAACAGGGTCCAA |
| Pou5f1 (RT-qPCR) | GACACCTGGCTTCAGACTTC | TCAGGCTGCAAAGTCTCCAC |
| Sox2 (RT-qPCR) | AGTGGTACGTTAGGCGCTTC | GACCACGAAAACGGTCTTGC |
| Nanog (RT-qPCR) | TGATTCAGAAGGGCTCAGCAC | AAGGCTGCAGAAAGTCCTCC |
| Scp3 (RT-qPCR) | AAATCTGGGAAGCCACCTTTG | CAATTTTCTGGTTACTGGCTTTG |
| Dmc1 (RT-qPCR) | GCGGCTACTCAGGTGGAAAG | AGTCGTGACAACATCTGGGC |
| Spo11 (RT-qPCR) | TGCTGTGCCGACTAACATTC | CGTAGGGATCTGCATCGAC |
| Sox8 (bp −139) | CGAAAGTCAAGCCGTGCTGC | AATCCAAAGAAGCCTTGCGAC |
| Sox8 (bp −500 to −700) | CTCTCCGCCTTCTCTCTAGG | CTGGCAGTGACTGCTGAGTC |
| Sox8 (bp −800) | CACGGGCTTGGGAAACAGGT | GAGGCGAGAGCCAAGCCTG |
a
Beta-actin gene.