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. 2015 Dec;13(4):3–9. doi: 10.15171/ijb.1234

Table 1. Designing of primers and probe to detect DNA-A, alphasatellite and betasatellite .

DNA-A: Amplicon size 182 bp Tm: 55°C
CP-F
CP-R
CP-P
5'AAACAACAGGCATGGACAAA'3,
5'CCGACACCACGAGTAACATC '3
Fam-5'TGGGCCTTCACAACCCTTTGG '3-Tamra
Alphasatellite:Amplicon size 192 bp: 55°C
Alpha Rep-F
Alpha Rep-R
Alpha Rep-P
5'GTCTTCCGACGAGTTAAGGC '3
5'GTCTCTGGCAAAGGTGGATT '3
Fam-5'AACGGGACCCAGATGACCGC '3-Tamra
Betasatellite: Amplicon size 186 bp: 55°C
BetaC1-F
BetaC1-R
BetaC1-P
5'TTCCTATTCGCATACAACGG '3
5'ATGCATTGCTGGTTTGTGTT '3
Fam-5'ACGGTTCGATTACATCCATTCCCAA'3-Tamra