Table 1. Designing of primers and probe to detect DNA-A, alphasatellite and betasatellite .
DNA-A: Amplicon size 182 bp Tm: 55°C | |
CP-F CP-R CP-P |
5'AAACAACAGGCATGGACAAA'3, 5'CCGACACCACGAGTAACATC '3 Fam-5'TGGGCCTTCACAACCCTTTGG '3-Tamra |
Alphasatellite:Amplicon size 192 bp: 55°C | |
Alpha Rep-F Alpha Rep-R Alpha Rep-P |
5'GTCTTCCGACGAGTTAAGGC '3 5'GTCTCTGGCAAAGGTGGATT '3 Fam-5'AACGGGACCCAGATGACCGC '3-Tamra |
Betasatellite: Amplicon size 186 bp: 55°C | |
BetaC1-F BetaC1-R BetaC1-P |
5'TTCCTATTCGCATACAACGG '3 5'ATGCATTGCTGGTTTGTGTT '3 Fam-5'ACGGTTCGATTACATCCATTCCCAA'3-Tamra |