Figure 5.

Differences in the TCR repertoire of CD4 and CD8 T cells specific to BTV VP7. A VP7 amino acid sequence of BTV VP7 protein showing the previously described T cell epitopes [18]. Striped boxes indicate MHC-I epitopes and dotted boxes indicate MHC-II epitopes. The name of each epitope is indicated with VP and the position of the first amino acid for each epitope in VP7. B PBMC isolated from BTV-infected sheep at different days post-infection were stimulated with individual peptides (indicated on each graph) and the amount of IFN-γ produced by T cells was evaluated by ELISA. * p < 0.05, ** p < 0.01; Mann-Whitney test (timepoints vs day 0).