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. 2017 Jun 29;48:38. doi: 10.1186/s13567-017-0444-3

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Inflammatory response during BTV infection. A CD14⁺ cells were quantified by flow cytometry from total PBMC obtained from BTV-infected sheep. B RNA was extracted from PBMC from BTV-infected sheep at different times post-infection. Reverse transcription to cDNA and IL-6, IL-1β, IL-12 and CXCL10 expression was assessed by RT-qPCR. The line represents the average gene expression in 4–8 animals tested at each time point. The arrows indicate time of infection with BTV-8. Data were normalized to β-actin expression and to pre-infection values (t = 0) (2^−∆∆Ct method). Student t test (time point vs t = 0); * p ≤ 0.05, ** p ≤ 0.01.