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. 2017 Jun 30;8:452. doi: 10.3389/fphys.2017.00452

Figure 4.

Figure 4

Effect on phrenic nerve activity of unilateral microinjection of AOAA into the preBötC of anesthetized, paralyzed rats breathing control gas (25% O2, balance N2). Representative recording of phrenic nerve activity showing the effects of local HEPES (A) or AOAA (B; 1 mM, 250 nL) microinjection into the preBötC on nerve output and ventilatory output for the same animal (vertical arrow in (C) represents onset of HEPES or AOAA injection) (C). Box plots of group data showing the time course of changes in burst frequency (D), burst amplitude (E) and ventilatory output (F) evoked by unilateral preBötC AOAA (1 mM, 250 nL, n = 10). The symbol *indicates difference (p < 0.05) compared to baseline.