Fig. 3.

Inhibition of CK2 leads to a reduction in BTIC frequency and proliferation. a X456 (20,000/well) or b X12 (15,000/well) cells were cultured with CX-4945 (5, 10 μM) or vehicle control for 72 h and proliferation assessed using the WST-1 assay (n = 3). Representative photos (20× magnification) of c X456 and d X12 neurospheres 6 days after plating in the absence or presence of CX-4945 (10 μM). White bar equals 200 μM. X12 cells cultured with CX-4945 (10 μM) or vehicle control for 3 h or 7 days were assessed for viability e, frequency of CD133⁺ stem cells f and total number of CD133⁺ cells, using flow cytometry. Fractions of viable cells and CD133⁺ cells were normalized to DMSO control at 3 h or 7 days (n = 3). g X12 cells were assessed for expression of Sox2 by flow cytometry after 7 days of culture with CX-4945 (10 μM) or vehicle control (n = 3). *p < 0.05