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[35S]methionine labeling of TK proteins produced using the reticulocyte lysate system. KOS, m51, m77, m83, m175, and DM21 are recombinant proteins of the respective mutants. Luciferase is the positive control of the reaction. A 15-μl sample of each product of the transcription-translation reaction was electrophoresed on an SDS-12% polyacrylamide gel and autoradiographed after 48 h.
