FIG. 2.

TK proteins produced using the bacterial system. (A) Western blot analysis of TK proteins. A 0.5-μg sample of each purified TK was loaded onto an SDS-10% polyacrylamide gel, transferred, and reacted with polyclonal anti HSV1-TK. (B) Standardization of the quantity of recombinant proteins. A 0.5-μg sample of each purified TK was electrophoresed on an SDS-12% polyacrylamide gel. The gel was stained with Coomassie blue. WT indicates TK from wild-type HSV-1 is the positive control; KOS, m51, m77, m83, m175, and DM21 are recombinant proteins of the respective mutants; DM21 is the negative control; L indicates the DNA ladder.