FIG. 3.

Enzymatic activities of TK proteins. (A) TK proteins produced using the reticulocyte lysate system. (B) TK proteins produced using the bacterial system. KOS is the positive control, and DM21 is the negative control. m51, m77, m83, m175, and DM21 are recombinant proteins of the respective mutants. Reticulocytes are the control of residual activity (reaction without any plasmid DNA to transcribe). Means and standard deviations (error bars) are calculated from three independent experiments performed for each mutant.