FIGURE 6.

Sitagliptin specifically impaired the RANKL-induced ERK and AKT cascade and suppressed the RANKL-induced activation of NFATc1 and c-Fos. (A,D) BMMs cells were treated with or without 50 μg/mL sitagliptin, followed by treatment with 50 ng/mL RANKL for the indicated periods. Cell lysates were subjected to Western blot analysis. The band intensities corresponding to p-ERK (B), p-AKT (C), NFATc1 (E), and c-Fos (F) were quantified, normalized relative to that of GAPDH, and converted to the fold change of the control. Representative images of three repeated experiments are shown. Data are expressed as mean ± SD; ^∗P < 0.05, ^∗∗P < 0.01 versus the RANKL-induced group.