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Effect of c-di-GMP on S. aureus cell-to-cell aggregation. (A and B) Results for a 24-h culture of strain DK825 treated with 200 μM c-di-GMP (A) and for an untreated control (B) are shown. (C and D) The results of Gram staining of c-di-GMP treated cells (C) and untreated cells (D) are shown. (E and F) The results obtained with a c-di-GMP-treated culture of bap mutant M556 (E) and an untreated control (F) are shown. Magnification in panels C and D is ×630.
