Table 2.
Molecular characteristics and epidemiological analysis of XDR K. pneumoniae clinical isolates in Wenzhou, 2013–2015 (n = 35).
| Isolate/year | Resistance determinants | Epidemiological analysis | ||||||
|---|---|---|---|---|---|---|---|---|
| Carbape-nemases | Other β-lactamases genes | Quinolone resistance genes | Aminoglycoside resistance determinants | Size of blaKPC−2 plasmid (kb) | Transcon-jugant | Replicon types | STs | |
| FK 688/13 | — | blaCMY, blaDHA−1 | — | AAC(6′)-Ib, APH(3′)-Ia | — | — | 1,525 | |
| FK 729/13 | KPC-2 | blaCTX−M−14, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | — | — | IncFrepB | 11 |
| FK 782/13 | KPC-2 | blaCTX−M−65, blaSHV−11 | qnrS | AAC(6′)-Ib | — | — | — | 290 |
| FK 1186/14a | KPC-2 | blaCTX−M−65, blaSHV−11 | qnrS, gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | 54.7 | J1186 | IncFrepB | 11 |
| FK 1271/14 | KPC-2 | — | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | — | — | IncFrepB | 11 |
| FK 1425/14 | KPC-2 | blaCTX−M−14, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, | — | — | IncFrepB | 11 |
| FK 1468/14a | KPC-2 | blaCTX−M−14, blaSHV−11 | qnrB, gyrA (S83I, D87G) | AAC(6′)-Ib, AAC(3)-IV | 54.7 | J1468 | — | 2,281 |
| FK 1668/14 | KPC-2 | blaCTX−M−14, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | — | — | IncFrepB | 11 |
| FK 1743/14a | KPC-2 | blaCTX−M−65, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | 167.1 | J1743 | IncFrepB | 11 |
| FK 1855/14 | KPC-2 | blaCTX−M−14, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | — | — | IncFrepB | 11 |
| FK 1869/14 | KPC-2 | blaCTX−M−65, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | — | — | IncFrepB | 11 |
| FK 1919/15 | KPC-2 | blaCTX−M−14, blaSHV−11 | aac(6′)-Ib-cr, gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | — | — | IncFrepB | 11 |
| FK 2048/15 | KPC-2 | blaCTX−M−65, blaSHV−11 | qnrS, parC (S80I) | AAC(6′)-Ib | — | — | IncFrepB | 11 |
| FK 2104/15 | KPC-2 | blaCTX−M−65, blaSHV−11 | aac(6′)-Ib-cr, gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | — | — | IncFrepB | 11 |
| FK 2152/15a | KPC-2 | blaCTX−M−15, blaSHV−11 | qnrS | AAC(6′)-Ib | 54.7 | J2152 | — | 268 |
| FK 2200/15 | KPC-2 | blaCTX−M−65, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | — | — | IncFrepB | 11 |
| FK 2203/15 | KPC-2 | blaCTX−M−15 | — | AAC(6′)-Ib | — | — | — | 14 |
| FK 2206/15 | KPC-2 | blaTEM−1, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | — | — | IncFrepB | 11 |
| FK 2219/15 | KPC-2 | blaCTX−M−14 | qnrB, gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | — | — | IncFrepB | 11 |
| FK 2267/15 | KPC-2 | blaCTX−M−65, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | — | — | IncFrepB | 11 |
| FK 2302/15a | KPC-2 | blaCTX−M−15, blaCTX−M−65, blaTEM−1, blaSHV−11 | qnrS, gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | 54.7 | J2302 | IncFrepB | 11 |
| FK 2322/15a | KPC-2 | blaCTX−M−14 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | 54.7 | J2322 | — | 11 |
| FK 2346/15a | KPC-2 | blaCTX−M−65, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | 54.7 | J2346 | IncFrepB | 11 |
| FK 2348/15 | KPC-2 | blaCTX−M−65, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | — | — | IncFrepB | 11 |
| FK 2578/15 | KPC-2 | blaCTX−M−14, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | — | — | IncFrepB | 11 |
| FK 1881/15a | KPC-2 | blaCTX−M−65, blaTEM−1, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | 54.7 | J1881 | IncFrepB | 11 |
| FK 1905/15 | KPC-2 | blaCTX−M−14, blaTEM−1, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, AAC(3)-IV, rmtB | — | — | IncFrepB | 11 |
| FK 1934/15 | — | blaSHV−11, blaDHA−1 | aac(6′)-Ib-cr, gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, AAC(3)-IV, rmtB | — | — | — | 11 |
| FK 1944/15 | KPC-2 | blaCTX−M−65, blaTEM−1, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, rmtB | — | — | IncFrepB | 11 |
| FK 2076/15a | KPC-2 | blaCTX−M−65, blaTEM−1 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | 54.7 | J2076 | — | 11 |
| FK 2016/15 | KPC-2 | blaCTX−M−14, blaTEM−1, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | — | — | IncFrepB | 11 |
| FK 2047/15a | KPC-2 | blaCTX−M−65, blaTEM−1, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6')-Ib, APH(3′)-Ia, AAC(3)-IV, rmtB | 54.7 | J2047 | IncFrepB | 11 |
| FK 2078/15 | KPC-2 | blaCTX−M−65, blaTEM−1 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | — | — | — | 11 |
| FK 2142/15a | KPC-2 | blaCTX−M−65, blaTEM−1, blaSHV−11 | gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | 54.7 | J2142 | IncFrepB | 11 |
| FK 2180/15a | KPC-2 | blaCTX−M−14, blaTEM−1, blaSHV−11 | qnrB, aac(6′)-Ib-cr, gyrA (S83I, D87G), parC (S80I) | AAC(6′)-Ib, APH(3′)-Ia, rmtB | 54.7 | J2180 | — | 656 |
a
Strains that have successfully transferred carbapenem resistance genes blaKPC to E. coli J53; “—” means undetected.