Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Generation of the ΔMovps41 mutant. (A) The split-marker approach was used to replace the entire MoVPS41 gene with the hygromycin resistance cassette (hph). Probe A is a PCR product amplified with primers AF (VPS41-AF) and AR (VPS41-AR). N: NcoI. (B) Southern blotting analysis with NcoI-digested genomic DNA of wild-type strain (Guy11), ΔMovps41 mutant strains (ΔMovps41-18, ΔMovps41-33, and ΔMovps41-34) and complemented transformant (C-21).
