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. 2017 Jun 30;8:1091. doi: 10.3389/fpls.2017.01091

FIGURE 5.

FIGURE 5

The ΔMovps41 mutant produced dysfunctional hyphal tip appressorium-like structures at hyphal tips. (A) Appressorium-like structures formed on hydrophobic film by hyphal tips of wild-type (Guy11), ΔMovps41 mutant (ΔMovps41-18) and complemented strain (C-21). Scale bar = 50 μm. (B) Bar graph representing quantification of the number of hyphae producing appressoria-like structures in the ΔMovps41 mutant, wild-type and C-21. Asterisks represent significant difference between Guy11 and ΔMovps41 mutant (P < 0.01). (C) Barley leaves inoculated with culture blocks of the ΔMovps41 mutant, wild-type and C-21 were examined for the formation of invasive hyphae at 72 hpi. Arrows indicate appressorium-like structures. Scale bar = 20 μm. (D) Quantification of collapsed appressorium-like structures in the ΔMovps41 mutant, wild-type Guy11 and C-21 strain. 2M and 5M glycerol were used for testing. Asterisks represent significant difference between Guy11 and ΔMovps41 mutant (P < 0.01).