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. 2017 Jun 30;8:778. doi: 10.3389/fimmu.2017.00778

Figure 6.

Figure 6

2-DG, DON, or oligomycin potently prevent IL-15-induced activation of TEMRA CD8. (A,B) Expression of CD25 (A) or CD69 (B) by TEMRA and effector memory (EM) CD8 T cells stimulated with plate-bound anti-CD3 (2 µg/mL) and IL-15 (10 ng/mL) for 24 h in the presence of 2-DG, oligomycin, or DON. Representative flow data are shown and bars indicate mean ± SEM of pooled data (n = 5–7). (C) Percentage of CPDeFluor405low cells within Annexin V− cells after 5 days of culture of purified CD8 subsets with plate-bound anti-CD3 (2 µg/mL) and IL-15 (10 ng/mL) in the presence of medium control or selective inhibitors (2-DG, oligomycin, DON). Representative flow data are shown and bars indicate mean ± SEM of pooled data (n = 5–7). Kruskal–Wallis test followed by a Dunn’s Multiple Comparison Test was performed (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).