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2-AA tolerization decreases the acetylation of NF-κBp65. Immunoblot showing decreased NF-κBp65K310 acetylation in 2-AA-tolerized RAW264.7 cells (A). HDAC1 KD restored NF-κBp65K310 acetylation in 2-AA-tolerized cells following 2-AA stimulation (B). Data are representative of three independent experiments. An asterisk (∗) indicates significant difference from untreated cells (P < 0.05). DU, densitometry units, bars represent SD.
