Figure 2.

Fangchinoline promotes the apoptosis of melanoma cells. (A and B) A375 and A875 cells were treated with fangchinoline for 24 h and stained with Hoechst 33258. (C and D) A375 and A875 cells were treated with fangchinoline for 24 h and Annexin V-FITC/PI staining was used to detect the percentage of apoptotic cells. (E and F) A375 cells were treated with the indicated concentration of fangchinoline for 24 h, the indicated proteins and mRNA levels were detected by western blot analysis and real-time PCR. Data are shown as mean ± SEM. **P<0.01 vs. DMSO treated group.