Figure 3.

Fangchinoline inhibits metastasis of melanoma cells. (A and B) A375 cells were treated with fangchinoline for 24 h; Transwell assay with or without Matrigel was performed. Cells were counted and results represent the mean ± SD of three experiments. **P<0.01 vs. DMSO treated group. (C and D) A375 cells were treated with fangchinoline for 24 h; Transwell assay with or without Matrigel was performed. Cells were counted and results represent the mean ± SD of three experiments. **P<0.01 vs. DMSO treated group. (E and F) A375 cells were treated with the indicated concentration of fangchinoline for 24 h, the indicated proteins and mRNA levels were detected by western blot analysis and real-time PCR. Data are shown as mean ± SEM. **P<0.01 vs. DMSO treated group.