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Determination of the IC50 of compound 3b against L. chagasi-infected macrophages. Pentavalent antimony was used as a standard drug. Macrophages were treated for 120 h at 37°C with drugs, and the number of infected macrophages in Giemsa-stained glass coverslips was determined by light microscopy. Dose-response curves were obtained in GraphPad Prism 3.0 software. Data are mean and standard deviation.
