Figure 4.

Characterization of the 5′ and 3′ junctions of the knock-in at the otx2 locus. Representative gel pictures of the otx2:venus (A) and otx2:tRFP (B) knock-in alleles from one founder (lanes 1 and 3) or wild-type siblings (lanes 2 and 4). Lanes 1 and 2 show the 5′ junction PCR (primer pair A+B from Figure 1) and lanes 3 and 4 show the 3′ junction (primers C+D from Figure 1). (C) DNA sequence analysis of the 5′ and 3′ junctions and mutations; “−” denotes deletion and “+” denotes insertion; inserted base pairs are marked in blue.