Skip to main content
. 2017 Jun 30;10:206. doi: 10.3389/fnmol.2017.00206

FIGURE 2.

FIGURE 2

Increased cellular clustering and IBA1 immunoreactivity in the white matter of the aging brain. (A) IBA1 staining coupled to Luxol fast blue staining in young and aged brains. Comparison of the density of microglia in the cortex, hippocampus and white matter regions, such as corpus callosum (CC), anterior commissure (AC), cerebellar white matter, and dorsal fornix; (B) Mac-2 staining together with luxol fast blue staining show increased Mac-2 positivity in the white matter of aged compared to young brain. Increased Mac-2 positivity is observed in white matter tracts including corpus callosum, anterior commissure, and cerebellar white matter; (C) Quantification of IBA1 positive pixels in different regions of young (n = 4) and old brains (n = 4). (D) Quantification of IBA1 positive pixels in different regions of young (n = 4) and old brains (n = 4). Scale bar: (A,B) = 25 μm. For, (C,D) Student’s t-test ∗P < 0.05, ∗∗P < 0.005, ∗∗∗P < 0.0005, ns, not significant. Error bars indicate standard deviation (SD).