Table 1.
Identified proteins from hBMECs targeted by Mpr1 grouped according to biological function.
| Protein name | Accession number | Molecular weight (kDa) | Sc <CnMpr1> + hCMEC/D3 | ScWT + hCMECD3 | Fold change |
|---|---|---|---|---|---|
| CYTOSKELETON AND MEMBRANE REARRANGEMENT | |||||
| Cluster of Myosin-9 | sp|P35579|MYH9_HUMAN | 227 | 6,602 | 1,301 | 6.9 |
| Cluster of Actin, cytoplasmic 2 | sp|P63261|ACTG_HUMAN | 42 | 667 | 230 | 3.9 |
| Cluster of Tubulin beta chain | sp|P07437|TBB5_HUMAN | 50 | 269 | 74 | 4.9 |
| Cluster of Tubulin alpha-1B chain | sp|P68363|TBA1B_HUMAN | 50 | 143 | 33 | 5.8 |
| Cluster of Myosin light polypeptide 6 | tr|G8JLA2|G8JLA2_HUMAN | 17 | 127 | 25 | 6.6 |
| Cluster of Tight junction protein ZO-2 | sp|Q9UDY2|ZO2_HUMAN | 134 | 122 | 7 | 24 |
| Cluster of Talin-2 | sp|Q9Y4G6|TLN2_HUMAN | 272 | 86 | 4 | 29 |
| Cluster of Unconventional myosin-Ic | sp|O00159|MYO1C_HUMAN | 122 | 82 | 6 | 19 |
| Cluster of Alpha-actinin-1 | sp|P12814|ACTN1_HUMAN | 103 | 30 | 17 | 2.5 |
| Cluster of Moesin | sp|P26038|MOES_HUMAN | 68 | 61 | 9 | 9.6 |
| Tight junction protein 1 (Zona occludens 1), isoform CRA_a | tr|G3V1L9|G3V1L9_HUMAN | 197 | 63 | 4 | 21 |
| Cluster of Filamin-A | sp|P21333|FLNA_HUMAN | 281 | 53 | 1 | 36 |
| Cluster of Annexin A2 | sp|P07355|ANXA2_HUMAN | 39 | 46 | 4 | 16 |
| Cluster of Unconventional myosin-Ib | sp|O43795|MYO1B_HUMAN | 132 | 42 | 2 | 28 |
| Cluster of Myosin regulatory light chain 12B | sp|O14950|ML12B_HUMAN | 20 | 38 | 12 | 3.9 |
| Heat shock protein beta-1 | sp|P04792|HSPB1_HUMAN | 23 | 26 | 1 | 35 |
| Profilin-1 | sp|P07737|PROF1_HUMAN | 15 | 36 | 1 | 49 |
| Cluster of F-actin-capping protein subunit beta | sp|P47756|CAPZB_HUMAN | 31 | 29 | 2 | 20 |
| Cluster of Cofilin-1 | sp|P23528|COF1_HUMAN | 19 | 23 | 4 | 7.8 |
| Cluster of F-actin-capping protein subunit alpha-1 | sp|P52907|CAZA1_HUMAN | 33 | 28 | 2 | 19 |
| Ras GTPase-activating-like protein IQGAP1 | sp|P46940|IQGA1_HUMAN | 189 | 20 | 1 | 32 |
| Cluster of Actin-related protein 3 | sp|P61158|ARP3_HUMAN | 47 | 14 | 1 | 19 |
| CELLULAR TRANSPORTATION | |||||
| Cluster of Unconventional myosin-Ic | sp|O00159|MYO1C_HUMAN | 122 | 82 | 6 | 19 |
| Major vault protein | sp|Q14764|MVP_HUMAN | 99 | 66 | 2 | 45 |
| Cluster of Endoplasmin | sp|P14625|ENPL_HUMAN | 92 | 51 | 8 | 6.2 |
| Cluster of Unconventional myosin-Ib | sp|O43795|MYO1B_HUMAN | 132 | 42 | 2 | 28 |
| Transitional endoplasmic reticulum ATPase | sp|P55072|TERA_HUMAN | 89 | 37 | 2 | 18 |
| Cluster of Clathrin heavy chain 1 | sp|Q00610|CLH1_HUMAN | 192 | 25 | 2 | 17 |
| METABOLIC PROCESS | |||||
| Cluster of Pyruvate kinase PKM | sp|P14618|KPYM_HUMAN | 58 | 96 | 17 | 7.6 |
| Alpha-enolase | sp|P06733|ENOA_HUMAN | 47 | 90 | 15 | 7.6 |
| Cluster of Fructose-bisphosphate aldolase A | sp|P04075|ALDOA_HUMAN | 39 | 68 | 6 | 16 |
| Cluster of Transketolase | sp|P29401|TKT_HUMAN | 68 | 58 | 6 | 13 |
| Cluster of L-lactate dehydrogenase A chain | sp|P00338|LDHA_HUMAN | 37 | 44 | 5 | 12 |
| Neutral alpha-glucosidase AB | sp|Q14697|GANAB_HUMAN | 107 | 33 | 1 | 45 |
| Triosephosphate isomerase | sp|P60174|TPIS_HUMAN | 31 | 26 | 4 | 8.8 |
| Cluster of ATP-dependent 6-phosphofructokinase, platelet type | sp|Q01813|PFKAP_HUMAN | 86 | 28 | 1 | 39 |
| Phosphoglycerate mutase 1 | sp|P18669|PGAM1_HUMAN | 29 | 20 | 3 | 9 |
| NUCLEOPROTEINS AND RIBONUCLEOPROTEIN AND PROTEIN SYNTHESIS | |||||
| Cluster of Nucleolin | sp|P19338|NUCL_HUMAN | 77 | 163 | 15 | 15 |
| Nucleophosmin | sp|P06748|NPM_HUMAN | 33 | 53 | 4 | 12 |
| Elongation factor 2 | sp|P13639|EF2_HUMAN | 95 | 51 | 5 | 2.8 |
| Heterogeneous nuclear ribonucleoprotein U | sp|Q00839|HNRPU_HUMAN | 91 | 39 | 2 | 26 |
| Heterogeneous nuclear ribonucleoprotein A1 | sp|P09651|ROA1_HUMAN | 39 | 48 | 6 | 11 |
| Cluster of Heterogeneous nuclear ribonucleoprotein Q | sp|O60506|HNRPQ_HUMAN | 70 | 23 | 1 | 31 |
| Heterogeneous nuclear ribonucleoproteins A2/B1 | sp|P22626|ROA2_HUMAN | 37 | 22 | 2 | 15 |
| Splicing factor U2AF 65 kDa subunit | sp|P26368|U2AF2_HUMAN | 54 | 15 | 4 | 5.4 |
| Heterogeneous nuclear ribonucleoprotein K | sp|P61978|HNRPK_HUMAN | 51 | 18 | 1 | 24 |
| Cleavage and polyadenylation specificity factor subunit 6 | sp|Q16630|CPSF6_HUMAN | 59 | 18 | 2 | 12 |
| IMMUNITY | |||||
| Cluster of Interferon-induced GTP-binding protein Mx2 | sp|P20592|MX2_HUMAN | 82 | 124 | 10 | 17 |
| Cluster of HLA class I histocompatibility antigen, A-11 alpha chain | sp|P13746|1A11_HUMAN | 41 | 44 | 3 | 20 |
| E3 ubiquitin-protein ligase TRIM21 | sp|P19474|RO52_HUMAN | 54 | 39 | 3 | 12 |
| Sequestosome-1 | sp|Q13501|SQSTM_HUMAN | 48 | 27 | 1 | 36 |
| PROTEIN FOLDING | |||||
| Cluster of Protein disulfide-isomerase A3 | sp|P30101|PDIA3_HUMAN | 57 | 40 | 10 | 5.4 |
| 78 kDa glucose-regulated protein | sp|P11021|GRP78_HUMAN | 72 | 48 | 3 | 6.9 |
| Peptidyl-prolyl cis-trans isomerase A | sp|P62937|PPIA_HUMAN | 18 | 39 | 8 | 6.6 |
| Protein disulfide-isomerase A6 | sp|Q15084|PDIA6_HUMAN | 48 | 17 | 4 | 5.7 |
| Cluster of Protein disulfide-isomerase | sp|P07237|PDIA1_HUMAN | 57 | 14 | 1 | 19 |
| CELL SIGNALING | |||||
| Cluster of Heat shock protein HSP 90-beta | sp|P08238|HS90B_HUMAN | 83 | 90 | 9 | 7.8 |
| Major vault protein | sp|Q14764|MVP_HUMAN | 99 | 66 | 2 | 45 |
| Calreticulin | sp|P27797|CALR_HUMAN | 48 | 35 | 11 | 4.8 |
| Cluster of Adenylyl cyclase-associated protein 1 | sp|Q01518|CAP1_HUMAN | 52 | 16 | 1 | 22 |
| DNA REPAIR | |||||
| Transitional endoplasmic reticulum ATPase | sp|P55072|TERA_HUMAN | 89 | 37 | 2 | 18 |
| Ubiquitin-like modifier-activating enzyme 1 | sp|P22314|UBA1_HUMAN | 118 | 17 | 1 | 8.5 |
Proteins were identified with a protein threshold >99.0% and ≥ 3 unique peptides of < 0.1 false discovery rate (FDR) using Scaffold 4. Proteins were categorized based on GO terms! associated with biological function and literature searches.