Fig. 3.

Humoral anti-PbCS responses in immunized mice. a Kinetics of anti-PbCS IgG responses elicited in mice immunized with 30, 150 and 300 YU of N-PbCS non-adjuvanted yeast lysates. Curves represent group mean value with SEM. OD_450nm are expressed in log₁₀ scale. “x3”—three bi-weekly immunizations; “x5”—five weekly immunizations. Mouse sera were analysed at 1/10³ dilution. b Isotyping of humoral IgG responses at day 42 in mice immunized with 30, 150, and 300 YU of N-PbCS non-adjuvanted yeast lysates. Bars correspond to median values per group. Asterisks indicate significant median differences (one symbol for p < 0.05, Mann–Whitney nonparametric test). Antibody titer of the non-parasitized mouse is encircled