Fig. 4.

Immunogenicity of N-PbCS at 30, 150 and 300 YU in yeast lysates. a Mean and standard deviations log₁₀ values of parasitaemia in mice immunized with N-PbCS at different doses and regimens and in non-immunized mice (naive) following infection with 10⁴ GFP⁺ P. berghei sporozoites. b Parasitaemia at day 5 post challenge. Bars correspond to medians. Decrease in parasitaemia median value in immunized groups in comparison with the naive group is indicated in folds. The non-parasitized mouse was excluded from calculation. c–f Groups of mice immunized with the following formulations and non-immunized mice from the same experiment: c 30 YU N-PbCS, N or WT yeast lysates 3 times bi-weekly; d 30 YU N-PbCS, N or WT yeast lysates 5 times weekly; e 150 YU N-PbCS, N or WT yeast lysates 5 times weekly; f 300 YU N-PbCS, N or WT yeast lysates 5 times weekly. Bars correspond to medians. NI not infected over 10 days post challenge (value below the limit of detection). Asterisks indicate significant median differences (one symbol for p < 0.05 and two for p < 0.005; Mann–Whitney nonparametric test). The non-parasitized mouse was excluded from calculation