Table 1.

Groups of mice, vaccine formulations, doses and administration protocols used in Experiments I, II and III

Group	Yeast lysate dose (YU)	Adjuvant	Number of mice	Number of immunizations
Experiment I
N-PbCS	30	No	8	5
PbCS	30a	No	8	5
N	30a	No	6	5
WT	30	No	6	5
Naive	/	/	8	/
Experiment II
N-PbCS	30	No	6	3
N-PbCS	30	No	6	5
N-PbCS	150	No	6	5
N-PbCS	300	No	6	5
N	30a	No	6	3
N	30a	No	6	5
N	150a	No	6	5
N	300a	No	6	5
WT	30	No	4	3
WT	30	No	4	5
WT	150	No	4	5
WT	300	No	4	5
Naive	/	/	8	/
Experiment III
N-PbCS	50	Yes	6	3
N-PbCS	100	Yes	6	3
N-PbCS	150	Yes	6	3
N	50a	Yes	6	3
N	100a	Yes	6	3
N	150a	Yes	6	3
WT	150	Yes	6	3
Alum	/	Yes	6	3
Naive	/	/	6	/

aDiluted with WT yeast according to the following calculation:
	Expression level, ng/YU	Mol. weight, g/mol	Expression level, pmol/YU	Folds diluted with WT to match N-PbCS	Antigen dose in 30 YU, ng
N-PbCS	12	91,32	131	/	360
PbCS	16	33,24	481	3,7	130
N	870	58,08	14,979	114,0	230

1 YU = 10⁷ cells