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. 2005 Mar;49(3):963–972. doi: 10.1128/AAC.49.3.963-972.2005

FIG. 1.

FIG. 1.

Budded-to-hyphal-form transition and its inhibition by latrunculin A. (A) Microplate-based morphological assay. (B) SC5314 cells grown in YNB medium were transferred to 384-well optical microplates containing either Spider medium, YPD plus 10% fetal calf serum, Lee's medium, or M199 pH 8 tissue culture medium and incubated at 37°C for 4 h. The percentage of hyphae observed is shown above each bar. (C) SC5314 cells grown in YNB medium were transferred to 384-well optical micoplates containing Spider medium and either latrunculin A (1.6 or 0.1 μM) or amphotericin B (0.2 or 0.02 μg/ml) and incubated at 37°C for 4 h. The percentage of hyphae observed is shown in the lower left of each photomicrograph.