Table 3.

Respiratory activities in membranes from KF707 W.T. and oxidase mutant cells grown, until the stationary phase (OD_{600 nm}) 0.7–0.9, in glucose (Gc) as sole carbon source.

Electron donors	NADH			ASCORBATE
Additions	/	${\text{N}}_3^{-}$	CN−	Cyt C	TMPD	TMPD/${\text{N}}_3^{-}$	TMPD/CN−
STRAINS
KF707 W.T.	165 ± 10	72 ± 3.3	29 ± 5.0	33 ± 1.0	157 ± 20	32 ± 3.0	7.0 ± 2.0
KFΔcox1-2	178 ± 20	38 ± 8.0	34 ± 10	59 ± 6.0	221 ± 14	21 ± 2.5	16 ± 1.0
KFΔcco1-2	175 ± 7.0	160 ± 2.0	125 ± 1.0	9 ± 0.5	38 ± 5.0	32 ± 3.3	8.0 ± 1.0
KFΔcox1-2/cco1-2	174 ± 15	172 ± 15	167 ± 15	1.0 ± 0.2	11 ± 2.0	11 ± 2.0	11 ± 2.0
KFΔCIO	145 ± 30	29 ± 5.0	1.0 ± 0.2	37 ± 3.5	208 ± 31	35 ± 3.5	10 ± 0.5
KFΔcox1-2/CIO	145 ± 5.0	25 ± 5.0	2.0 ± 1.0	45 ± 7.0	163 ± 12	10 ± 2.5	10 ± 2.5
KFΔcco1	196 ± 7.0	165 ± 2.0	110 ± 3.0	26 ± 2.5	124 ± 5.0	12 ± 3.0	3.0 ± 1.0
KFΔcco2	220 ± 10	78 ± 5.0	51 ± 5.0	33 ± 2.5	145 ± 5.0	37 ± 3.5	9.0 ± 2.0

Symbols and abbreviations used: ${\text{N}}_{\mathit{\text{3}}}^{-}$, sodium azide (30 μM); CN⁻, potassium cyanide (50 μM); Cyt C, horse-heart cytochrome C (50 μM); TMPD, N,N,N′,N′-Tetramethyl-p-phenylene diamine (50 μM). Mutant phenotype strains abbreviations as in Table 1. Rates, expressed as nmoles of O₂ consumed min⁻¹ mg of proteins⁻¹, are the mean of at least two/three membrane preparations from independent cell cultures.