Table 4.

Respiratory activities in membranes from KF707 W.T. and oxidase mutant cells grown, until the stationary phase (OD_{600 nm}) 0.7–0.9, in biphenyl (Bp) as sole carbon source.

Electron donors	NADH			ASCORBATE
Additions	/	${\text{N}}_3^{-}$	CN−	CytC	TMPD	TMPD/${\text{N}}_3^{-}$	TMPD/CN−
STRAINS
KF707 W.T.	111 ± 2.5	64 ± 9.5	7.0 ± 0.3	37 ± 2.0	165 ± 13	88 ± 15	7.0 ± 2.0
KFΔcox1-2	38 ± 5.0	9.0 ± 3.3	3.0 ± 0.2	17 ± 3.0	75 ± 10	5.0 ± 2.0	3.0 ± 2.0
KFΔcco1-2	96 ± 9.3	80 ± 12	29 ± 0.4	18 ± 3.0	117 ± 12	108 ± 5.0	6.0 ± 1.7
KFΔcox1-2/cco1-2	36 ± 5.0	35 ± 5.0	35 ± 5.0	2.0 ± 1.0	6.0 ± 2.5	6.0 ± 2.5	6.0 ± 2.5
KFΔCIO	83 ± 4.0	60 ± 5.0	4.0 ± 2.0	37 ± 0.4	174 ± 1.0	110 ± 5.0	12 ± 1.0
KFΔcox1-2/CIO	104 ± 4.0	15 ± 2.5	3.5 ± 1.5	8.3 ± 3.5	67 ± 1.0	9.3 ± 2.0	8.3 ± 2.0
KFΔcco1	111 ± 8.0	97 ± 7.0	37 ± 2.0	20 ± 2.5	201 ± 18	150 ± 15	20 ± 7.0
KFΔcco2	95 ± 5.0	46 ± 5.0	11 ± 3.0	25 ± 3.0	200 ± 20	114 ± 13	13 ± 5.0

Symbols and abbreviations used: ${\text{N}}_{\mathit{\text{3}}}^{-}$, sodium azide (30 μM); CN⁻, potassium cyanide (50 μM); CytC, horse heart cytochrome C (50 μM); TMPD, N,N,N′,N′-Tetramethyl-p-phenylene diamine (50 μM). Mutant phenotype strains abbreviations as in Table 1. Rates, expressed as nmoles of O₂ consumed min⁻¹ mg of protein⁻¹, are the mean of at least two/three membrane preparations from independent cell cultures.