Figure 6.

Overexpression of PinX1 induces apoptosis of MDA-MB-231 cells in vitro. (A and B) Western blot analysis was used to measure the expression levels of cleaved caspase-3 in different experimental groups. GAPDH was a loading control. (C) Flow cytometric analysis (left) of the apoptotic rate of the PinX1-overexpressed group with its negative control and the PinX1 knockout group with its counterpart. Quantitative analyses of the apoptotic rate on the right. At least three independent experiments were preformed (*P<0.05, two-tailed unpaired t-test) and the data expressed are mean ± SEM.