TABLE 1.
DNA sequencing and real-time PCR data for MDR M. tuberculosis strains from Germany, stratified for Beijing and non-Beijing strainsa
| MDR strain group (no. of strains) | Affected rpoB codon(s) | Nucleotide/amino acid change(s) | Affected katG, inhA, or ahpC codon | Nucleotide/amino acid change(s) | No. (%) of strains |
|---|---|---|---|---|---|
| All (103) | 531 | TCG→TTG/Ser→Leu | katG 315 | AGC→ACC/Ser→Thr | 64 (62.1) |
| TCG→TTG/Ser→Leu | None | 3 (2.9) | |||
| TCG→TTG/Ser→Leu | katG 315 | AGC→ACA/Ser→Thr | 2 (1.9) | ||
| TCG→TTG/Ser→Leu | katG 315 | AGC→AAC/Ser→Asn | 2 (1.9) | ||
| TCG→TTG/Ser→Leu | inhA 209 | C→T | 2 (1.9) | ||
| TCG→TTT/Ser→Phe | katG 315 | AGC→ACC/Ser→Thr | 2 (1.9) | ||
| TCG→TGG/Ser→Trp | katG 315 | AGC→ACC/Ser→Thr | 1 (1.0) | ||
| TCG→TGG/Ser→Trp | inhA 209 | C→T | 1 (1.0) | ||
| TCG→TGG/Ser→Trp | None | 1 (1.0) | |||
| 526 | CAC→AAC/His→Asn | katG 315 | AGC→ACC/Ser→Thr | 4 (3.9) | |
| CAC→CTC/His→Leu | katG 315 | AGC→ACC/Ser→Thr | 3 (2.9) | ||
| CAC→TAC/His→Tyr | katG 315 | AGC→ACC/Ser→Thr | 2 (1.9) | ||
| CAC→CGC/His→Arg | katG 315 | AGC→ACC/Ser→Thr | 2 (1.9) | ||
| CAC→CGC/His→Arg | ahpC-oxyR | C(−52)T | 1 (1.0) | ||
| CAC→GAC/His→Asp | katG 315 | AGC→ACC/Ser→Thr | 1 (1.0) | ||
| CAC→TGC/His→Cys | katG 315 | AGC→ACC/Ser→Thr | 1 (1.0) | ||
| 516 | GAC→GTC/Asp→Val | katG 315 | AGC→ACC/Ser→Thr | 2 (1.9) | |
| GAC→TAC/Asp→Tyr | katG 315 | AGC→ACC/Ser→Thr | 1 (1.0) | ||
| 522 | TCG→CAG/Ser→Gln | katG 315 | AGC→ACC/Ser→Thr | 1 (1.0) | |
| TCG→TTG/Ser→Leu | None | 1 (1.0) | |||
| 518 | AAC→ATC/Asn→Ile | None | 1 (1.0) | ||
| 513 | CAA→CCA/Gln→Pro | None | 1 (1.0) | ||
| 517 | Delb | katG 315 | AGC→ACC/Ser→Thr | 1 (1.0) | |
| 514-516 | Delb | katG 315 | AGC→ACC/Ser→Thr | 1 (1.0) | |
| 176 | GTC→TTC/Val→Phe | ahpC-oxyR | G(−48)A | 1 (1.0) | |
| 519/522/531 | Delb, TCG→TTG/Ser→Leu, and TCG→TTG/Ser→Leu | katG 315 | AGC→ACC/Ser→Thr | 1 (1.0) | |
| Beijing (62) | 531 | TCG→TTG/Ser→Leu | katG 315 | AGC→ACC/Ser→Thr | 48 (77.4) |
| TCG→TTG/Ser→Leu | inhA 209 | C→T | 1 (1.6) | ||
| TCG→TTG/Ser→Leu | None | 1 (1.6) | |||
| TCG→TTT/Ser→Phe | katG 315 | AGC→ACC/Ser→Thr | 2 (3.2) | ||
| 526 | CAC→AAC/His→Asn | katG 315 | AGC→ACC/Ser→Thr | 2 (3.2) | |
| CAC→CTC/His→Leu | katG 315 | AGC→ACC/Ser→Thr | 2 (3.2) | ||
| CAC→TAC/His→Tyr | katG 315 | AGC→ACC/Ser→Thr | 2 (3.2) | ||
| CAC→CGC/His→Arg | katG 315 | AGC→ACC/Ser→Thr | 1 (1.6) | ||
| 518 | AAC→ATC/Asn→Ile | None | 1 (1.6) | ||
| 516 | GAC→GTC/Asp→Val | katG 315 | AGC→ACC/Ser→Thr | 1 (1.6) | |
| 519/522/531 | Delb, TCG→TTG/Ser→Leu, and TCG→TTG/Ser→Leu | katG 315 | AGC→ACC/Ser→Thr | 1 (1.6) | |
| Non-Beijing (41) | 531 | TCG→TTG/Ser→Leu | katG 315 | AGC→ACC/Ser→Thr | 16 (39.0) |
| TCG→TTG/Ser→Leu | katG 315 | AGC→ACA/Ser→Thr | 2 (4.9) | ||
| TCG→TTG/Ser→Leu | katG 315 | AGC→AAC/Ser→Asn | 2 (4.9) | ||
| TCG→TTG/Ser→Leu | inhA 209 | C→T | 1 (2.4) | ||
| TCG→TTG/Ser→Leu | None | 2 (4.9) | |||
| TCG→TGG/Ser→Trp | katG 315 | AGC→ACC/Ser→Thr | 1 (2.4) | ||
| TCG→TGG/Ser→Trp | inhA 209 | C→T | 1 (2.4) | ||
| TCG→TGG/Ser→Trp | None | 1 (2.4) | |||
| 526 | CAC→AAC/His→Asn | katG 315 | AGC→ACC/Ser→Thr | 2 (4.9) | |
| CAC→GAC/His→Asp | katG 315 | AGC→ACC/Ser→Thr | 1 (2.4) | ||
| CAC→CGC/His→Arg | katG 315 | AGC→ACC/Ser→Thr | 1 (2.4) | ||
| CAC→CGC/His→Arg | ahpC-oxyR | C(−52)T | 1 (2.4) | ||
| CAC→TGC/His→Cys | katG 315 | AGC→ACC/Ser→Thr | 1 (2.4) | ||
| CAC→CTC/His→Leu | katG 315 | AGC→ACC/Ser→Thr | 1 (2.4) | ||
| 522 | TCG→CAG/Ser→Gln | katG 315 | AGC→ACC/Ser→Thr | 1 (2.4) | |
| TCG→TTG/Ser→Leu | None | 1 (2.4) | |||
| 516 | GAC→GTC/Asp→Val | katG 315 | AGC→ACC/Ser→Thr | 1 (2.4) | |
| GAC→TAC/Asp→Tyr | katG 315 | AGC→ACC/Ser→Thr | 1 (2.4) | ||
| 513 | CAA→CCA/Gln→Pro | None | 1 (2.4) | ||
| 517 | Delb | katG 315 | AGC→ACC/Ser→Thr | 1 (2.4) | |
| 514-516 | Delb | katG 315 | AGC→ACC/Ser→Thr | 1 (2.4) | |
| 176 | GTC→TTC/Val→Phe | ahpC-oxyR | G(−48)A | 1 (2.4) |