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Effect of simocyclinone D8 on Ca2+- and ciprofloxacin (CFX)-induced DNA cleavage by gyrase. Increasing concentrations of simocyclinone D8 (left-hand panels) were incubated in the presence of fixed concentrations of Ca2+ (4 mM) or ciprofloxacin (5 μM). Controls with novobiocin (Novo) are shown on the right. Gels were run in the presence of 1 μg of ethidium bromide per ml. C, control (no enzyme or drug); E, enzyme only; NC, nicked circle; L, linear; R, relaxed.
