Figure 7.
Impedance measurements (a) and conventional measurements obtained using the 96-well plate/fluorescence method (b) for the adhesion of K. pneumoniae KCTC 2242 and of its isogenic mutants to mucin modified surfaces. For impedance measurements, normalized impedance change (NIC) at 0.1 Hz was used to measure the adhesion of bacteria to mucin surfaces. Increases in impedance signals were proportional to number of bacteria in the range from 105 to 108 CFU/mL K. pneumoniae KCTC 2242 ∆wabG showed the lowest level of interaction with mucin surfaces. Mean values with different superscripts (a,b) at each bacterial concentration indicate significant differences between the wild type and its isogenic mutant strains (p < 0.05).